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Saturday, May 16, 2020 | History

2 edition of Cellular and biochemical analysis of induced sputum found in the catalog.

Cellular and biochemical analysis of induced sputum

John Vincent Fahy

Cellular and biochemical analysis of induced sputum

a novel method for studying airway inflammation and mucus hypersecretion in asthmatic subjects

by John Vincent Fahy

  • 391 Want to read
  • 32 Currently reading

Published .
Written in English

    Subjects:
  • Asthma.,
  • Airway Obstruction.

  • Edition Notes

    StatementJohn Vincent Fahy.
    ID Numbers
    Open LibraryOL22840778M

    The cellular profile of sputum in normal subjects and in patients with asthma and COPD is different. The concentration of eosinophils in the sputum correlates with the severity of asthma. Figures. Sputum induction was successful in 96 subjects (81% of the recruited subjects). The induced sputum samples had a mean weight (SD, range) of mg (93, to mg) with cell viability of 70% (17, 23 to 96%). The selected sputum was mucoid and was minimally contaminated with saliva as indicated by a mean squamous cell count of 4% (SD 6%).

    Abstract. Bronchoalveolar lavage fluid (BALF) and sputum, obtained through more or less noninvasive techniques, contain cells and many soluble compounds from respiratory tract secretions and are, therefore, important sources for the study of the lung and its pathologies. Duration of inhalation. The duration of inhalation is another important variable in sputum induction. At least two studies have reported that the cellular and biochemical constituents of induced sputum change during the course of sputum induct These studies found that neutrophils and eosinophils are prominent in samples collected early during sputum induction, whereas lymphocyte .

    Cellular and biochemical analysis of induced sputum from asthmatic and from healthy subjects. Am Rev Respir Dis ; – OpenUrl PubMed Web of Science. Analysis of induced sputum has been adopted over the ential cell count in sputum. It has been suggested that, irrespective of the technique used, changes could occur in the cellular and biochemical composition of sequential samples collected throughout the induction procedure [5, 6]. Although these preliminary data have not all been con-.


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Cellular and biochemical analysis of induced sputum by John Vincent Fahy Download PDF EPUB FB2

To determine the feasibility of cellular and biochemical analysis of sputum induced after inhalation of hypertonic (3%) saline, we analyzed sputum induced in 10 healthy and in 18 asthmatic subjects. We also analyzed saliva samples from all by: Cellular and biochemical analysis of induced sputum from asthmatic and from healthy subjects.

Fahy JV(1), Liu J, Wong H, Boushey HA. Author information: (1)Department of Medicine, University of California, San Francisco Cited by: Total cell counts and cell differentials of nonsquamous cells in induced sputum samples were determined after the samples were homogenized in dithiothreitol.

Centrifugation of the entire homogenized sputum sample yielded supernatant that could be analyzed for biochemical by: These changes are in close agreement with previously described allergen-induced changes in airway bronchoalveolar lavage fluid.

We conclude that analysis of the cellular and biochemical constituents of induced sputum is a useful means of studying acute changes in allergic inflammation in Cited by: Analysis of cellular and biochemical constituents of induced sputum after allergen challenge: a method for studying allergic airway inflammation.

Fahy JV(1), Liu J, Wong H, Boushey HA. Author information: (1)Department of Medicine, University of California, San Francisco Cited by: Homogenized sputum samples could be loaded in acceptable numbers on Chipcytometry slides.

The cell load protocol had to be optimized to sputum cells and requires a minimum attachment time of 15 min. (Fig. Chipcytometry analysis up to 9 days of storage at 4°C, showed that >98 % of the sputum cells remained on the chip following 6 staining and.

The separate analysis of induced sputum from three consecutive sampling periods of a single induction procedure demonstrated significant changes in their cellular and biochemical composition, both in healthy and mild asthmatic subjects.

Fahy, J. V., Liu, J., Wong, H., and Boushey, H. () Cellular and biochemical analysis of induced sputum from asthmatic and from healthy subjects. In recent years, induced sputum has became a new re-search tool in diagnostics of occupational asthma, which provides a better understanding of the pathogenesis of this disease.

Sputum induction by inhalation of hypertonic saline is a non-invasive method of obtaing secretions from the lower respiratory tract for cell counting [2]. Summary Sputum induction is a relatively non-invasive mode of airway sampling that provides an opportunity for analysis of cellular components and infective agents, including bacteria and viruses, together with fluid-phase constituents.

Both induction and processing of sputum samples are standardised and several manuals are available to help to educate professionals to perform the Cited by: To determine the feasibility of cellular and biochemical analysis of sputum induced after inhalation of hypertonic (3%) saline, we analyzed sputum induced.

JW Fahy, J Lim, H Wong, et ar and biochemical analysis of induced sputum from asthmatic and from healthy subjects Am Rev Respir Dis, (), pp. Google Scholar. Fahy, J. V., Liu, J., Wong, H., and Boushey, H. () Cellular and biochemical analysis of induced sputum from asthmatic and from healthy subjects.

Rev. TY - JOUR. T1 - Induced sputum analysis. T2 - Step by step. AU - Weiszhar, Zsoka. AU - Horvath, Ildiko. PY - /6/1. Y1 - /6/1. N2 - Sputum induction is a relatively non-invasive mode of airway sampling that provides an opportunity for analysis of cellular components and infective agents, including bacteria and viruses, together with fluid-phase constituents.

Sputum induction has recently been proposed as the only direct noninvasive or relatively noninvasive method for measuring airway inflammatory indices ().In subjects with asthma and/or chronic bronchitis, the numbers and types of cells present in induced sputum differ from those in the sputum of healthy subjects, suggesting that evaluation of induced sputum may be a reproducible and valid.

Cell Biochemistry and Biophysics fosters progress in comprehending the nature of the biochemical and biophysical mechanisms underlying the control of cellular physiological homeostasis and the consequences of its perturbation.

computer analysis of tissues, cells, cell networks, organelles, and molecular/macromolecular assemblies; research.

Fahy JV, Liu J, Wong H, Boushey HA () Cellular and biochemical analysis of induced sputum from asthmatic and from healthy subjects. Am Rev. Elevated B cells in sputum of asthmatics.

Close correlation with eosinophils. Am J Respir Crit Care Med. Feb; (2)– Fahy JV, Liu J, Wong H, Boushey HA. Analysis of cellular and biochemical constituents of induced sputum after allergen challenge: a method for studying allergic airway inflammation.

J Allergy Clin by: The use of induced sputum, together with pulmonary function tests, makes it possible to monitor the pattern of inflammation more appropriately in specific respiratory conditions.

(14,15) However, total and differential cell counts in induced sputum samples should be delimited by normal reference values. Book. Jul ; James Paton Analysis of cellular and biochemical constituents of induced sputum after allergen challenge: A method for studying allergic airway inflammation We conclude.

Conclusions: The duration of sputum induction significantly affects the cellular and biochemical composition of induced sputum in a manner suggesting that large airways are sampled at the beginning of sputum induction, whereas peripheral airways and alveoli are sampled at later time periods. Our data demonstrate the importance of standardizing the duration of sputum induction in clinical research .analysis of spontaneously produced or induced sputum has recently been suggested for repeated evaluation of airway inflammation in patients with asthma [6–8], chronic bron-chitis [7–9] and COPD [9].

The aim of the present study was to assess the success rate, reproducibility and safety of a method for sputum induction in patients with severe.Since the first attempts to use standardised methods for sampling induced airways sputum, two methods for processing the expectorate have evolved.

The first involves selecting all viscid or denser portions from the expectorated sample with the aid of an inverted microscope 1, 2. This method has been extensively evaluated and reported in detail 2–4.